Secondary Detection & Chromogen

The HiDef Detection™ Polymer Systems are high-sensitivity visualization systems that are ready-to-use in immunohistochemical protocols. This two-step system uses an indirect method resulting in an antibody-enzyme complex that universally detects primary mouse and rabbit antibodies. The resulting chromogenic reaction can be visualized by either HRP or Alk Phos compatible chromogens using light microscopy. They are biotin-free and eliminate non-specific staining that could result from any endogenous biotin. These visualization systems consist of two detection reagents for amplifying the detection of low expressing antigens within a shorter turnaround time. These systems are compatible with both manual and automated staining platforms (subject to available software-selectable options in the latter instances).